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Image Search Results
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: Soluble factors released by activated cytotoxic T lymphocytes interfere with death receptor pathways in neuroblastoma
doi: 10.1007/s00262-007-0412-2
Figure Lengend Snippet: Supernatant from activated CTLs both induce caspase-8 expression and modulate its activity in NB cells. a NB cell lines were treated for 48 h with either medium alone, or in the presence of 10% v/v of control supernatant (CS) or activated supernatant (AS). The expression of caspase-8 in total cell lystaes was monitored by Western blot using a specific antibody recognizing the 55/50 kD pro-form of caspase-8 as well as the 40/36 and 23 kD cleavage products obtained upon activation. Expression of actin was used as a control of sample loading. b The activity of caspase-8 in NB cells propagated for 24, 48 and 72 h in complete medium alone (thin open histograms), or in the presence of either CS (filled histograms) or AS (bold open histograms) was measured by flow cytometry using fluorescent caspase-8 specific substrates as described in Materials and Methods. Numbers show percentage of AS-treated cells positive for active caspase-8
Article Snippet: The following antibodies and reagents were used for Western blot analysis: the mouse monoclonal antibody specific to β-actin was purchased from Sigma Chemical Co (St Louis, USA) and the
Techniques: Expressing, Activity Assay, Western Blot, Activation Assay, Flow Cytometry
Journal: EMBO Molecular Medicine
Article Title: An immunohistochemical atlas of necroptotic pathway expression
doi: 10.1038/s44321-024-00074-6
Figure Lengend Snippet: Reagents and tools table
Article Snippet: A range of working dilutions were trialed for the following antibodies, although no conditions could be optimized for specificity and intensity: rabbit anti-phospho-RIPK1 (clone D813A; RRID:AB_2799268; Cell Signaling Technology Cat#44590 S); mouse anti-RIPK1 (clone 38/RIP; RRID:AB_397831; 0.25 g/L BD Biosciences Cat#610459); mouse anti-RIPK1 (clone 334640; RRID:AB_2253447; 0.5 g/L; R&D Systems Cat#MAB3585); rabbit anti-RIPK3 (clone 18H1L23; RRID: AB_2866471; 0.5 g/L; Thermo Fisher Scientific Cat#703750); rabbit anti-phospho-RIPK3 (clone D6W2T; RRID:AB_2800206; Cell Signaling Technology Cat#93654); rabbit anti-phospho-MLKL (clone D6E3G; RRID:AB_2799112; Cell Signaling Technology Cat# 37333); rabbit anti-phospho-MLKL (clone EPR9514; RRID:AB_2619685; Abcam Cat#ab187091; (Wang et al, )); mouse anti-MLKL (clone 3D4C6; RRID:AB_2882029; 1.957 g/L; ProteinTech Cat#66675-1-IG); rat anti-MLKL (clone 3H1; RRID:AB_2820284; 2 g/L produced in-house (Murphy et al, ) and available from Millipore Cat# MABC604); rabbit anti-mouse MLKL (clone D6W1K; RRID:AB_2799118; Cell Signaling Technology Cat#37705); rabbit anti-MLKL (clone 2B9; RRID:AB_2717284; 1 g/L; Thermo Fisher Scientific Cat#MA5-24846); mouse anti-Caspase-10 (clone 4C1; RRID:AB_590721; 1 g/L; MBL International Cat# M059-3);
Techniques: Derivative Assay, Polymer, Plasmid Preparation, Blocking Assay, Membrane, Staining, RNA HS Assay, dsDNA Assay, Protease Inhibitor, Software, Imaging, Enzyme-linked Immunosorbent Assay